Journal of Gastrointestinal Infections

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VOLUME 11 , ISSUE 1 ( January-December, 2021 ) > List of Articles

ORIGINAL RESEARCH

Study of Prevalence of Campylobacter Gastroenteritis among Pediatric Population Using a Multiplex PCR in a Tertiary Care Hospital in Puducherry, South India

Lakshmi Shanmugam, Jharna Mandal, Niranjan Biswal, Noyal M Joseph

Keywords : Campylobacter, Children, Gastroenteritis, Multiplex PCR

Citation Information : Shanmugam L, Mandal J, Biswal N, Joseph NM. Study of Prevalence of Campylobacter Gastroenteritis among Pediatric Population Using a Multiplex PCR in a Tertiary Care Hospital in Puducherry, South India. J Gastrointest Infect 2021; 11 (1):9-14.

DOI: 10.5005/jp-journals-10068-3050

License: CC BY-NC 4.0

Published Online: 18-01-2022

Copyright Statement:  Copyright © 2021; The Author(s).


Abstract

Background and objectives: Campylobacter is one of the four common causes of diarrheal illness worldwide. Campylobacter infection is more common in developing than in developed countries. As conventional methods pose a great difficulty for the isolation and identification of this organism, molecular methods are much preferred. Materials and methods: A total of 133 stool samples were collected from children less than 13 years presenting to pediatric outpatient department and the emergency department in a tertiary care hospital in Puducherry. The stool samples were extracted, and the DNA was subjected to multiplex PCR to detect Campylobacter species, followed by sequencing. Results: Campylobacter species was detected in 13 children (9.7% of the study population) (95% CI: 5.5–16.4), with Campylobacter jejuni (11 of 13) being the predominant species. The prevalence was higher in children less than 24 months (18.18%) with a higher predisposition to girls (14.29%). The most common clinical presentation was found to be acute watery diarrhea (10%). Campylobacter detection was higher from August to November (62%), with the highest incidence in October (22.3%). Campylobacter was detected in six (21.4%) children who had contact with pets. Conclusion: The study reveals that the prevalence of Campylobacter infection was high in Puducherry. There is an increased need to implement molecular assays for the routine detection of Campylobacter in all clinical pediatric stool samples.


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