Background: Stress, depression, and anxiety range between 10% and 12% of mental disorders. Despite this, mental health in India is heavily stigmatized and not frequently discussed. Work-related stress plays a big part in either causing or exacerbating mental health illnesses which in turn affects the complete well-being. Aims and objectives: The study aims to assess depression, anxiety, and stress levels among information technology (IT) employees. Materials and methods: An institution-based cross-sectional design was conducted among IT employees. A standardized psychological screening tool—Depression Anxiety Stress Scale 21 (DASS-21) was used. The data were analyzed for central tendencies as well as for any associations and correlations. Results: The study showed that around 54% of the workers had a positive score for anxiety and 18% of the workers had a positive score for stress and depression on the DASS-21 scale. The association between gender was statistically not significant whereas the association between age group with stress and overall DAS (depression, anxiety, stress) was statistically significant. Conclusion: The study found a prevalence rate of around 18–54% of depression, anxiety, and stress among the IT employees.