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MGM Journal of Medical Sciences

Article Details

2015 | January-March | Issue1
Original Article
Biological Synthesis of Antimicrobial Silver Nanoparticles by Phaseolus vulgaris Seed Extract
Nilesh S Paul, Revati Sharma, Raman P Yadav
Author Affiliation
Nilesh S Paul : MGM School of Biomedical Sciences, Navi Mumbai, Maharashtra, India

Revati Sharma : MGM Medical College and Hospital Navi Mumbai, Maharashtra, India

Raman P Yadav : MGM Medical College and Hospital, MGM Institute of Health Sciences, Navi Mumbai, Maharashtra, India

10.5005/jp-journals-10036-1036

ABSTRACT

Biological synthesis of silver nanoparticles is generally a time-consuming process in comparison to chemical process. Despite voluminous reports on biological synthesis of silver nanoparticles, there is still a challenge to develop fast synthesis of nanoparticles in the range of minutes/seconds through biological route. Several disadvantages are generally being posed by slow biological synthesis of silver nanoparticles including cost of operation. To overcome this difficulty, fast and simple method has been developed for the synthesis of silver nanoparticles, using Phaseolus vulgaris seed extract simply by increasing the temperature. The method is very quick and the color change of the reaction can be observed within 20 seconds. This process was able to synthesize silver nanoparticles within 80 seconds at 100oC which was confirmed by absorption peak at 413.79 nm in UV-visible spectrum. Initially, it was observed that P. vulgaris seed extract was unable to synthesize silver nanoparticles at 37oC even after 24 hours. The silver nanoparticles generated by this method were predominantly spherical in shape and in the range of approximately 4 to 30 nm in size, as characterized by transmission electron microscopy (TEM). On FTIR analysis, it was found that the nanoparticles possessed definite surface exposed groups. Generated silver nanoparticles showed antimicrobial activity against clinical isolates, Escherichia coli and Candida albicans. Thus, this biological process offers a simple, ecofriendly and very fast synthesis of antimicrobial silver nanoparticles.

Keywords: Silver nanoparticles, Very fast synthesis, Biological synthesis, Antimicrobial, Phaseolus vulgaris seed.

How to cite this article: Paul NS, Sharma R, Yadav RP. Biological Synthesis of Antimicrobial Silver Nanoparticles by Phaseolus vulgaris Seed Extract. MGM J Med Sci 2015; 2(1):1-6.

Source of support: Nil

Conflict of interest: None

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