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MGM Journal of Medical Sciences

Article Details

2014 | July-September | Issue3
REVIEW ARTICLE
Hand-made Cloning: A Guide for Cloning Water Buffaloes
SK Singla, A Raja, N Nala, MS Chauhan, RS Manik, P Palta
Author Affiliation
SK Singla : Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

A Raja : Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

N Nala : Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

MS Chauhan : Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

RS Manik : Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

P Palta : Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

10.5005/jp-journals-10036-1022

ABSTRACT

The present review is a detailed discussion on comparable benefits of hand-made cloning (HMC) technique than micro-manipulation based conventional cloning and developed in the author’s laboratory. Hand-made cloning technique does not require micromanipulators, because the manipulations required for both enucleation and nucleus transfer are performed by hand. The HMC technique includes manual bisection of zona-free oocytes and the simultaneous fusion of the somatic cell with two cytoplasts to produce a cloned embryo. The benefits of HMC include low setup costs for limited equipment, no requirement of highly trained expertise and in vitro effciency comparable to traditional somatic cell nuclear transfer technology. Embryos produced by HMC can be cryopreserved and capable of producing live births. The HMC technique is now applied to different species and can be used in large scale nuclear transfer programs.

Keywords: Buffalo, Cloning, Hand-guided cloning, Somatic-cell.

How to cite this article: Singla SK, Raja A, Nala N, Chauhan MS, Manik RS, Palta P. Hand-made Cloning: A Guide for Cloning Water Buffaloes. MGM J Med Sci 2014;1(3):126-131.

Source of support: Nil

Conflict of interest: None

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