Article Details

2014 | January-March | Issue1
Original Article
Toxicity Assessment of Silver Nanoparticles in Zebrafish Embryos
Mansee Thakur, Navami Dayal, Girish Pai, Dattatraya Joshi
Author Affiliation
Mansee Thakur : MGM Institute of Health Sciences and MGM College of Engineering and Technology, Navi Mumbai, Maharashtra, India

Navami Dayal : MGM Institute of Health Sciences, Navi Mumbai, Maharashtra, India

Girish Pai : MGM Institute of Health Sciences and MGM College of Engineering and Technology, Navi Mumbai, Maharashtra, India

Dattatraya Joshi : MGM Institute of Health Sciences, Navi Mumbai, Maharashtra, India

10.5005/jp-journals-10036-1003

ABSTRACT

Genotoxic effects of silver nanoparticles (Ag-np) in a vertebrate model system were investigated.
Effects and accumulation patterns of silver nanoparticles were studied using zebrafish embryos. Nanoparticles of silver were synthesized by chemical reduction of silver nitrate, using sodium borohydride as reducing agent and polyvinyl pyrolidene as a stabilizer. These nanoparticles were characterized by UV/ Vis spectrophotometer (absorption spectra), Transmission electron microscopy and were found to have the size range of 4 to 10 nm. Evaluation of cytotoxicity was carried out at various concentrations to obtain the LD50 value. Dose dependent decrease in percent viability was observed on exposure of embryos to different concentrations of silver nanoparticles with LD50 of 1.0 µg/ml. The results indicate that silver nanoparticles induce a dose-dependent toxicity in embryos and abrogate normal development.

Keywords: Zebrafish embryos, Silver nanoparticles, 4 to 10 nm, TEM, UV/Vis spectrophotometer.

How to cite this article: Thakur M, Dayal N, Pai G, Joshi D. Toxicity Assessment of Silver Nanoparticles in Zebrafish Embryos. MGM J Med Sci 2014;1(1):13-17

Source of support: Nil

Conflict of interest: None

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