Aim: Dhumrapatra is an important medicinal plant used in Indian system of medicine. Due to overexploitation and unsystematic collection, it is becoming rare and endangered in some parts of India. To meet the demand of the authentic drug and propagate the plant on a large scale, an in vitro propagation technique has been developed for Dhumrapatra.

Materials and methods: The collected plant was identified with the help of floras. Murashige and Skoog basal (MS) medium was prepared as per the standard protocol. Surface sterilization of explants was done with the help of disinfectant and 0.1% solution of HgCl2. Media were autoclaved at 121°C under 15 lb/inch2 pressure for 15 minutes. Cultures were incubated at 22°C ± 2°C and 8 hours photoperiod with light intensity of 3000 lux. Observations were recorded after a 4-week period.

Results: Best establishment of shoots (20 nos.) was found in MS media augmented with 0.5 mg/L kinetin (Kn). Maximum roots (2-3 nos) produced in MS were fortified with 3 mg/L indolebutyric acid (IBA). Maximum number of shoots and roots were achieved on nodal explants inoculated on MS + Kn (0.5 mg/L) + 0.1% polyvinylpyrrolidone (PVP).

Conclusion: The in vitro propagation protocol developed for Dhumrapatra would be beneficial for rapid multiplication and conservation of important medicinal plant.

Keywords: Conservation, Dhumrapatra, Hormones, In vitro, Regeneration.

How to cite this article: Mhase AG, Sinha R, Gurav AM, Rao G, Mangal AK, Srikanth N. Micropropagation of Dhumrapatra (Aristolochia bracteolata Lam.): An Important Medicinal Plant of Ayurveda. J Drug Res Ayurvedic Sci 2018;3(1):9-14.

Source of support: Nil

Conflict of interest: None