The aim of the study was to evaluate the ability of four topical desensitizing agents on dentinal tubule impediment utilizing confocal laser scanning microscopy (CLSM). Buccal cervical areas of 45 extracted human molars were smoothed and wetcleaned with SiC paper, trialed by utilization of 17% ethylenediaminetetraacetic acid (EDTA) so as to mimic the clinical aspect of overly sensitive dentin of cervical surfaces.
The teeth were randomly divided into one control group (n = 5) and four experimental groups, as indicated by the dentin surface treatments: group I: GLU; group II: MSC; group III: NAN; group IV: TMD; group V: control. The samples were analyzed under the confocal laser examining magnifying lens. The extents of totally blocked, halfway impeded, and open tubules inside each group were calculated.
The ratios of totally and incompletely blocked tubules to the total tubules for all the groups were determined, and the data were statistically analyzed utilizing nonparametric tests and statistical significance was calculated. The depth of penetration was greatest for MS Coat (1.35), while it was least for Gluma (1.07). The difference among all the groups was not statistically significant for depth of penetration. Gluma desensitizer demonstrated all the more totally impeded tubules (0.44) as well as partially blocked tubules (0.37). The distinctions among all the groups were statistically significant for tubule impediment (p ≤ 0.05).

Keywords: Confocal laser microscopy, Dentinal hypersensitivity, Topical desensitizing agents.

How to cite this article: Chouksey P, Shankaregowda AM, Lingareddy UH, Paramashivaiah A, Lakshminarasimhaiah V, Lingaiah U. An in vitro Analysis of Different Desensitizing Agents applied on Human Dentin. Int J Prev Clin Dent Res 2018;5(1):5-10.

Source of support: Nil

Conflict of interest: None