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International Journal of Preventive and Clinical Dental Research


Introduction: Elimination of microorganisms from the root canal and preventing them from reinfecting is the main objective of endodontic treatment. The sealers exhibit antimicrobial activity that may contribute to the elimination of intracanal microorganisms and the success of endodontic treatment.

Aim: To evaluate the antibacterial and antifungal activities of different root canal sealers against endodontic pathogens. Setting and design: This in vitro study was carried out at the Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College & Hospital, Hassan, Karnataka, India.

Materials and methods: Agar diffusion method was used. A double-base layer of Mueller-Hinton agar was prepared. The suspensions of Enterococcus faecalis, Escherichia coli, and Candida albicans were inoculated on agar medium. Four wells of equal dimensions were prepared and immediately filled with freshly mixed zinc oxide eugenol-based (Tubli-Seal), epoxy-based (AH Plus), polymethacrylate-based (EndoREZ), calcium hydroxide-based (Sealapex) sealers according to the manufacturer’s instructions. All the plates were incubated at 37°C for 24 hours, and microbial inhibition zones formed around the wells were measured after 24, 48, and 72 hours. Statistical analysis: Data were statistically analyzed by one-way analysis of variance to compare the differences among four sealers.

Results: All the four sealers caused microbial growth inhibition. AH Plus showed the greatest mean microbial growth inhibition of 17 mm followed by EndoREZ. The Sealapex and Tubli-Seal comparatively exhibited mild antimicrobial activity.

Conclusion: It could be concluded that all the sealers used showed antimicrobial activity. The AH Plus and EndoREZ showed the highest antimicrobial activity against tested organisms.

Keywords: Agar diffusion method, AH Plus, Endodontic bacterial strains, EndoREZ, Sealapex, Tubli-Seal.

How to cite this article: Ramachandra PKM, Krishnegowda SC, Jaganath BM, Rudranaik S, Manjula CG, Kurup NB, Madanan S. in vitro Comparative Evaluation of the Antibacterial and Antifungal Activities of Different Root Canal Sealers against Endodontic Pathogens. Int J Prev Clin Dent Res 2016;3(4):261-266.

Source of support: Nil

Conflict of interest: None

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