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International Journal of Oral Care & Research


Introduction: The aim of the study is to evaluate the antimicrobial activity of AH Plus, Metaseal, Realseal SE, and EZ Fill root canal sealers against Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans.

Materials and methods: Microbiological assays were carried in a laminar flow chamber using a standard ATCC strain. The microorganisms were cultivated at 37°C for 24 hours. Bacterial suspension was prepared to match the turbidity equivalent to 1.0 McFarland standard tube. Ten replica plates containing brain heart infusion (BHI) agar, Sabouraud’s agar, and 20 plates containing Mueller Hinton agar were prepared. Four groups were made: Group I (E. faecalis), group II (S. aureus), group III (P. aeruginosa), and group IV (C. albicans). In each petri dish, five wells were made – AH Plus, Metaseal, Realseal SE, EZ Fill, and Control. The agar diffusion test was used for determining the zone of inhibition.

Results: In our study, control showed the maximum zone of inhibition against all microorganisms except P. aeruginosa. When the intragroup comparison of inhibition zones of all sealers in groups I, II, III, and IV were made, Metaseal showed larger zones of inhibition than EZ Fill, AH Plus, and Realseal SE.

Conclusion: All the tested groups of sealers except Realseal SE have shown some amount of zone of inhibition against all the tested microorganisms. After 24 hours, the maximum zone of inhibition was shown by Metaseal followed by EZ Fill and AH Plus, and the difference was statistically significant.

Keywords: Agar diffusion test, AH plus, Antimocrobial activity, E. Feacalis, Metaseal.

How to cite this article: Saini P, Kumar M, Taneja S, Srivastav V. Evaluation of Antimicrobial Efficacy of Root Canal Sealers against Endodontic Pathogens: An in vitro Study. Int J Oral Care Res 2016;4(4):267-272.

Source of support: Nil

Conflict of interest: None

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